Uidbim0429320 and norte2020programa operacional regional do norte norte010145feder000012. Bioproduction and optimization of rosmarinic acid production in solenostemon of high yielding clone via encapsulation. Influence of rosmarinic acid and salvia officinalis. Inthepresentwork,wereexaminedthee ects of summer savory extracts and thier rosmarinic acid rich. Research article rosmarinic acidrich extracts of summer. Abstract rosmarinic acid is a fundamental compound of rosmarinus officinalis. Rosmarinic acid figure 1c, an ester of caffeic acid and 3, 4dihydroxyphenyllactic acid, is commonly found in species of the boraginaceae, lamiaceae, and in some ferns and hornworts petersen, simmonds, 2003. Additionally, it can be absorbed through the skin when in an ethanol base. Rosmarinic acid is a phenolic compound and ester of caffeic acid, which is naturally occurring in several plants of the lamiaceae family, including rosemary rosmarinus officinalis, from which it was originally isolated, sage salvia officinalis and spanish sage salvia lavandulifolia, basil ocimum tenuiflorum, oregano origanum vulgare, marjoram origanum majorana, and.
Solvent extraction and purification of rosmarinic acid from supercritical fluid extraction fractionation waste. It may help fight herpes simplex virus as well as improve heart and lung function. Molecules free fulltext rosmarinic acid, a rosemary. Rosmarinic acid is one of the polyphenolic substances contained in culinary herbs such as perilla perilla. Rosmarinic acid is potent flavonoid with powerful antioxidant and antiinflammatory properties. Evaluation of the virucidal effects of rosmarinic acid. In this work, the intrinsic prooxidant generation of h 2 o 2 and free radicals by the action of peroxidase and antioxidant consumption of h 2 o 2 and free radicals properties of rosmarinic acid are characterized.
Recent advances in the analysis of rosmarinic acid from herbs in. The main components of the caffeic acid derivatives fraction of leaves of mentha piperita l. These fractions led to pure compounds via bioguided fractionations see section 2. Rosa is most commonly found in species of the boraginaceae and the subfamily nepetoideae of the lamiaceae. Sources of rosmarinic acid rosmarinic acid can be extracted from rosemary rosmarinus officinalis, a woody, perennial herb native to the mediterranean region which is a commonly used. However, it is also found in species of other higher plant families and in some fern and hornwort species. In addition, its melanogenic prooxidant activity is characterized as it acts as a substrate of. Raisabundantinseveralmedicinal plants of the lamiaceae family, such as rosemary rosmarinus officinalis l.
Quantification of chlorogenic acid, rosmarinic acid, and. Ra has numerous biological and pharmacological activities. Rosmarinic acid pharmaceutical and clinical aspects. Although it is mostly know for its ability to improve mood and memory, it has other benefits, too. Rosmarinic acid ra, an ester of caffeic acid and 3,4dihydrophenyllactic acid figure 1 fig. Rosemary, whose major caffeoylderived and diterpenoid ingredients are rosmarinic acid, carnosol, and carnosic acid, is an important source of natural antioxidants and is being recognized increasingly as a useful preservative, protectant, and even as a potential medicinal agent. Carnosic acid had stronger inhibition at lower dosages, however when reaching concentrations over 100 lgmg that rate of inhibition dropped. Cn103044253a extraction separation method of rosmarinic. Despite the fact that several peaks appear in the tea chromatogram, the rosmarinic acid peak is always the major peak and appears with t r 7.
Skeletal muscle is a major insulintarget tissue and plays an important role in glucose homeostasis. Rosmarinic acid is an ester of caffeic acid and 3, 4dihydroxyphenyllactic acid. Rosmarinic acid, derived from rosemary a member of the mint. Recent studies on rosmarinic acid and its biological and. Rosmarinic acid accumulation is shown in hornworts, in the fern family blechnaceae and in species of several.
Chemically, rosmarinic acid is a caffeic acid ester of 33,4dihydroxyphenyllactic acid natural occurrences. The above chart shows that rosmarinic acid was able to inhibit the formation of ages by 97. N2 a thinlayer chromatographic tlcdensitometric method was used for the parallel quantification of rosmarinic acid ra and caffeic acid ca in crude extracts of salvia species family lamiaceae, obtained by ultrasonic extraction with 60% methanol. Recent studies on rosmarinic acid and its biological ncbi. Rosmarinic acidrich extracts of summer savory satureja. It is commonly found in species of the boraginaceae and the subfamily nepetoideae of the lamiaceae. Their structures were established by spectroscopic analysis mono and bidimensional nmr, ms and direct comparison with published data 14, 15 as rosmarinic acid ra, 0. Understanding the stability of these components and their mode of interaction in mixtures is important if they are. Among its numerous biologic functions, rosmarinic acid is known supporting a healthy eosiniphil response and healthy b and t.
Degradation study of carnosic acid, carnosol, rosmarinic. The invention is further directed to a process for producing the same and to pharmaceutical preparations containing the same. Studies on jamu and the medicinal resources in indonesia. Rosmarinic acid protects human dopaminergic neuronal cells against hydrogen peroxideinduced apoptosis.
Improvement effect of rosmarinic acid on serum testosterone. Phytochemical, pharmacological and pharmacokinetics effects of rosmarinic acid. Typical chromatograms for rosmarinic acid and melissa officinalis tea sample are shown in figures 2 and 3, respectively. The leaves have a mild lemon scent similar to mint. In this work, the intrinsic prooxidant generation of h2o2. Pdf solvent extraction and purification of rosmarinic. Rosmarinic acid is a constituent of the plant as well as a cosmetic ingredient. Rosmarinic acid content of fe and ae the concentration of rosmarinic acid in ae and fe was determined by hplc as previously described, with some modifications wang et al. First was to establish high rosmarinic acid line of in vitro sprout.
The aim of this study in healthy humans was to determine the absorption, metabolism, and urinary excretion of rosmarinic acid ra after a single intake of perilla extract pe. Rosmarinic acid and its methyl ester as antimicrobial. Rosmarinic acid was first isolated and characterized in 1958 by the italian chemists m. Lemon balm melissa officinalis, balm, common balm, or balm mint, is a perennial herbaceous plant in the mint family lamiaceae and native to southcentral europe, the mediterranean basin, iran, and central asia, but now naturalized in the americas and elsewhere it grows to a maximum height of 70150 cm 2859 in. Pdf phytochemical, pharmacological and pharmacokinetics. Rosmarinic acid extract for antioxidant, antiallergic, and. Rosmarinic acid is a plantbased compound found in a wide variety of spices, but most well known for being the active ingredient in rosemary and perilla oil. Rosmarinic acid ra, an ester of caffeic acid and 3,4dihydrophenyllactic acid figure 1, is a naturally occurring phenylpropanoid that is commonly found in species of the boraginaceae family, the subfamily nepetoideae of the lamiaceae family, and in lower plants such as ferns and hornworts petersen and simmonds, 2003. Rosmarinic acid, an ester of caffeic acid and 3,4dihydroxyphenyllactic acid, is a specialized plant metabolite that occurs in rather high quantities mainly in species of the subfamily nepetoideae of the lamiaceae and the boraginaceae, but also. Rosmarinic acid, a new snake venom phospholipase a2 inhibitor from cordia verbenacea boraginaceae. Impaired insulin action in muscles leads to insulin resistance and type 2 diabetes mellitus. The invention relates to an extraction separation method of rosmarinic acid in salvia castabea diels f.
Four compounds were detected as responsible for the major part of the antimicrobial activity of h. It is a redorange powder that is slightly soluble in water, but well soluble is most organic solvents. Search results for rosmarinic acid at sigmaaldrich. The antioxidant capacity as trolox equivalent antioxidant capacity teac and total phenolic contents, based on folinciocalteu reagent, in different ocimum species were quantitated. Phytochemical is produced when a herb reacts on environmental. Rosmarinic acid ra, an ester of caffeic acid and 3. Antioxidant activity, total phenolics, and rosmarinic acid. Rosmarinic acid is a phenolic compound and ester of caffeic acid, which is. Influence of nitrogen nutrition on growth and accumulation. Analysis of the virucidal effects of rosmarinic acid ra on ev71 infection. Effect of rosmarinic acid on immunological and neurological systems introduction rosmarinic acid rosa is an ester of caffeic acid and 3,4dihydroxyphenylactic acid figure 1. The effect of rosmarinic acid on immunological and. Validated hplcpda method for rosmarinic acid quantification in rosemary article pdf available in latin american journal of pharmacy 3010.
Ampactivated kinase ampk is an energy sensor, its activation increases glucose uptake in skeletal muscle and ampk activators have been viewed as a targeted approach in combating insulin. Pdf bioactivity and potential health benefits of rosmarinic acid. Quantitative hplc analysis of rosmarinic acid in extracts. Safety assessment of rosmarinus officinalis rosemary. However, previous studies of rosmarinic acid in cell models have yielded controversial results. Rosmarinic acid has a number of interesting biological. Rosmarinic acid the active ingredient from rosemary. While rosmarinus officinalis rosemaryderived ingredients are reported to have a number of functions, the most common. By the supramolecular complex which was formed from flavocommelin and perilla leaf extract as initial materials, the supernatant containing rosmarinic acid was isolated. Rosmarinic acid is a natural watersoluble antioxidant, with a variety of biological activity, widely used in food, medicine, and cosmetics. The effect of tea fermentation on rosmarinic acid and antioxidant. Dunal, in principles and practice of phytotherapy second edition, 20. The present invention is directed to a new rosmarinic acid phospholipidecomplex product containing rosmarinic acid and the phospholipide in a molar ratio of 1. Rosmarinic acid is a fundamental compound of rosmarinus officinalis.
It displays general antioxidant and antiinflammatory effects, and may protect against various forms of cancers. Clovamide and rosmarinic acid induce neuroprotective effects in in vitro models of neuronal death. The method comprises the following steps that salvia castabea diels f. Rosmarinic acid extract with potent biological activities was successfully isolated by supramolecular technique and solvent extraction from perilla leaves. Structurally speaking rosmarinic acid is a dimer of caffeic acid and 3,4dihydroxyphenyllactic acid, bound by an ester linkage and is known as a phenylpropanoid. Rosmarinic acid supplement health benefits, dosage, side. Rosmarinic acid is an ester of caffeic acid and 3,4dihydroxyphenyllactic acid.
Highperformance liquid chromatography hplc was used to identify and quantify rosmarinic acid, the. Rosmarinic acid ra is an ester of caffeic acid and 3,4dihydroxyphenyllactic acid which is one of the most frequently occurring caffeic acid esters in the plant kingdom besides chlorogenic acid. Rosmarinic acid and related metabolites springerlink. Oregano and other medicinal mint species have antioxidant properties which are now known to be due in large part to rosmarinic acid ra a compound with proven medicinal value and wellcharacterized physiological functions. Overview rosmarinic acid complex a very good defense against inflammatory ailments like cardiovascular disease, arthritis, and asthma.